Transgenic Mice

Two or more days of microinjection are required to complete the injection of up to 200 embryos with DNA. The injected embryos will be transferred to pseudo-pregnant recipient mice.  Implanted dams will be maintained in the facility until their pups can be weaned and delivered to the requesting investigator.  10-30% of the transferred embryos give rise to viable mice, and of these typically 20% carry the transgenic DNA in their germlines (i.e., about 5-10 transgenic founders should be expected).  The efficiency of generating transgenic mice using NOD, C57BL/6 mice or most inbred strains is lower than when using FVB/N or hybrid mice, thus the costs are higher for these experiments.  Investigators with special needs are encouraged to contact the core to discuss them.  Details concerning the preparation of DNA for microinjection can be found here.

Preparation of DNA for Making Transgenic Mice