BAC Mutagenesis by Homologous Recombination

Bacterial strains and reagents for manipulating bacterial artificial chromosomes can be obtained from the DERC mouse genetics core facility.  The core facility currently uses the ‘recombineering’ system developed in the laboratories of Drs. Donald Court, Neal Copeland, and Nancy Jenkins at the NCI in Frederick.  This system permits targeted mutagenesis of BACs (and other episomes) by homologous recombination.  Mutated BACs can be used as transgenes or as targeting vectors.  Check here for more detailed information about recombineering and to learn more about how to get permission to obtain the required bacterial strains.  After completing the simplified MTA process described on the website, and receiving permission from the NCI, you may acquire the bacterial strains from the DERC mouse genetics core facility.  Details of additional recombineering plasmids developed at UCSF will be posted here soon.

Recombineering Information